Production and Purification of the Thermophilic Bacteriophage TP-84

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Production and purification of the thermophilic bacteriophage TP-84.

A new procedure for production and purification of the thermophilic bacteriophage TP-84 in high yields is described. Cultures of Bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a pH of 6.5, were infected with the phage in a 100-liter fermentor. Addition of magnesium chloride (0.01 M) and a temperature of 58-C we...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1975

ISSN: 0003-6919

DOI: 10.1128/aem.29.2.219-223.1975